Table 3. Sera from macaques immunized with rBmHAT participated in the killing of B. malayi L3 in an ADCC mechanism.
| Animal I.D | Live L3 Mean ± S.Dof five wells | Dead L3 Mean ± S.Dof five wells | % Larval death Mean ± S.Dof five wells | Mean %Larval death |
| 4995 | 10 | 0 | 0 | 0 (Control) |
| 5240 | 10 | 0 | 0 | |
| 5249 | 10 | 0 | 0 | |
| 5252 | 10 | 0 | 0 | |
| 5253 | 10 | 0 | 0 | |
| 4996 | 7.5±0.5735 | 1.5±0.5735 | 25±5.16931* | 35% ±6.123724* (Immunized) |
| 5242 | 6.5±0.57735 | 4±0.57735 | 38±6.914* | |
| 5254 | 6.5±1 | 3±0.5735 | 31±7.3981* | |
| 5258 | 6.5±1.5275 | 5±0.57735 | 45±6.265021* | |
| 5259 | 7±1.154701 | 3.5±1.154701 | 35±11.54701* |
50 µl of sera samples from rBmHAT immunized macaques or control macaques were incubated with 2×105 PBMC from naive macaques and 10 B. malayi L3 for 48 hrs at 37°C. Larval viability was evaluated at the end of the incubation.
Significant larval death **(P<0.05) compare to other macaques. Control wells were L3 incubated with media, cells alone or sera alone.