Table 4. Detection of Mf in the macaques challenged with B. malayi L3.
Macaque groups | Animal ID | Presence of Mf | |
Knott test | PCR test | ||
Control (Immunized with only alum) | 4995 | + | − |
5240 | − | − | |
5249 | + | + | |
5252 | − | − | |
5253 | + | − | |
rBmHAT (Immunized with rBmHAT mixed with alum) | 4996 | + | + |
5242 | − | − | |
5254 | + | − | |
5258 | − | − | |
5259 | − | − |
The appearance of Mf in the peripheral blood of macaques during weeks 11–20 post challenge was detected by Knott and PCR techniques. 3 macaques in control group and 2 macaques in the immunized group were found to be microfilaremic.