Fig. 5.

Loss of cold-induced transcriptional repression of FLC is additive when vrn5 is combined with a TEX transgene. qRT-PCR analysis of unspliced FLC RNA through a time-course of cold exposure in Ler and vrn5-5 plants (A) and CTL, TEX lines or vrn5-5/TEX (B). Values are means ± SEM of three biological replicates. (C) Proposed model: COOLAIR negatively affects H3K36me3-methylation status and functions independently of PHD-PRC2–mediated H3K27 trimethylation to promote FLC silencing during vernalization.