Fig. 3.

Reconstitution of atomic models of Fat4 and Dachsous1. (A) Typing of CBMs in Fat4 and Dachsous1. CBM types 1–4 were defined according to the classification in Tables S1–S3. CBM type 5 corresponds to the EC–EC linkers with modified Ca²⁺-binding abilities. (B and C) Fitting of atomic models to TEM image of Fat4 (B) and Dachsous1 (C). TEM images of the molecule were overlaid with the best-fit atomic models shown in red. Out of 25 images analyzed for each molecule, two representative images were chosen, which are identical to those used in Fig. 1 C and D. Cyan and yellow circles indicate N and C termini of the models, respectively. “N” represents the putative N terminus of the molecule, inferred from its distal position of the linear strand. (Scale bar, 20 nm.) (D and E) Representative atomic models of Fat4 (D) and Dachsous1 (E). The models were drawn as representatives of the structures obtained through the analysis of 25 TEM images for each molecule.