Fig. 5.

Effects of FIIN-2, FIIN-3, and FRIN-3 on FGF- and EGF-induced dispersion of SKOV-3 cells in a 3D microfluidic device. Images show representative spheroids of SKOV-3 cells after indicated treatment with FGF (A) or EGF (B), in the presence or absence of the FIIN-2 and FIIN-3. Objective magnification power is indicated in the lower right corner. (A) Phase-contrast images of SKOV-3 spheroids at the indicated times after the addition of FGF. In contrast to the cell dispersal observed in control-treated devices, treatment with 1.0 μM of FIIN-2, FIIN-3, or FRIN-2 inhibited FGF1-induced dispersion of SKOV-3 cells. (B) Phase-contrast images of the SKOV-3 spheroids induced to disperse with EGF and subjected to control, FIIN-2, or FIIN-3 treatment. At 1.0 µM FIIN-3, but not FIIN-2, fully inhibited the EGF-induced dispersion of SKOV-3 cells.