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. 2014 Oct 28;111(45):16172–16177. doi: 10.1073/pnas.1418483111

Fig. 3.

Fig. 3.

HsfB2b is important for sustenance of period under high temperature. (A and B) Temperature compensation of hsfB2b-1 PRR7:LUC and HsfB2b-ox CCA1:LUC at 22 °C and 27 °C. Seedlings were entrained under 12L:12D at 22 °C for 1 wk and transferred to constant conditions at ZT0. The graphs show the mean period estimate of single seedlings vs. the mean RAE of the estimates. Low RAE indicates robust rhythmicity. Shown are RAE-weighted period means and SEM at 22 °C and 27 °C for hsfB2b-1 PRR7:LUC (A) and HsfB2b-ox CCA1:LUC (B). **P < 0.01 (Student t test) for 22 °C compared with 27 °C; n = 10–20. (C) tPRCs of HsfB2b-ox, prr7-3, and Col-0. Seedlings harbored the CCA1:LUC reporter and were entrained for one week (under 12L:12D at 22 °C) and then released in LL at 22 °C. Phase resetting pulses of 38 °C were applied for 3h at different times during the circadian cycle (circadian time, CT; corrected for period length). The circadian phase changes were measured based on the circadian phase of nonpulsed plants. Error bars represent SEM of technical replicates; n = 15–26.