Fig. 2.

Kif5C knockdown affects transport of protein cargos identified from proteomics. Immunohistochemical analyses of PURα, DIC1, and GluR1 (A–C) and bar graphs (D–F) showing quantitative analyses of immunohistochemical data shown in A–C. PURα/β-tubulin (A), DIC1/α-tubulin (B), and GluR1/β-tubulin (C) were coimmunostained with Hoechst (blue) in cultured hippocampal neurons with or without Kif5C siRNA transfection for 72 h. The fluorescence intensities of these protein cargos in neurites were quantified with Leica DM5500 software and statistically analyzed by one-way ANOVA, followed by Tukey’s post hoc test (D–F). KD, knockdown; NC, scrambled control siRNA; CON, vehicle-only control. Error bars represent SEM. ***P < 0.001. (Scale bar: 20 μm.)