Fig. 4.

OSR1 and SPAK regulate different WNK1 functions in HUVECs. (A) Proliferation of HUVECs over 72 h. Cell numbers are relative to time 0. (B) Migration through a membrane toward medium without or with 10% FBS. (C) Lysates from HUVECs treated as indicated were immunoblotted with anti-Slug, anti-cleaved PARP, and anti–β-actin. Blots were quantified via LI-COR imaging. (D) Secreted VEGF-A was measured by ELISA following a 2-h starvation. Data are mean ± SEM; n = 3 (A–C), n = 5 (D). *P < 0.05, ***P < 0.001.