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. 2014 Oct 31;111(45):15999–16004. doi: 10.1073/pnas.1419057111

Fig. 5.

Fig. 5.

Slug rescues cord formation by WNK1-depleted HUVECs. (A) Effect of Flag–Slug on cord formation by cells depleted of WNK1; representative images are from two experiments. (Scale bars, 100 μm.) (B) Lysates from HUVECs treated as indicated were immunoblotted with anti-Flag, anti-Slug, anti-WNK1, and anti–β-actin; n = 2. (C) Proliferation of cells treated as indicated over 72 h. Cell numbers are relative to time 0. (D) Migration of cells toward medium without or with 10% FBS. (E) Changes in expression of angiogenesis- and EMT-related genes in HUVECs treated as indicated. (F) Schematic diagram of the mechanisms of WNK1 actions on endothelial cell functions. Data are mean ± SD; n = 2 (C–E). **P < 0.01, ***P < 0.001.