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. 2014 Oct 29;111(45):16196–16201. doi: 10.1073/pnas.1417473111

Fig. 2.

Fig. 2.

NO promotes PLP accumulation through up-regulation of SNO1, a vitamin B6 salvage pathway enzyme. (A) PLP levels in wild-type and sno1 mutant. (B) Effect of SNP on PLP content in WT. (C) PLP content in nox1 mutant. (D) Expression analyses of SOS4 and PDX3 in response to SNP treatment. The 1-wk-old WT seedlings were transferred to the media containing indicated concentrations of SNP for 2 d and collected for the analysis. Data shown are means ± SD, n = 3. (E) Enzyme activity of SOS4 and PDX3 in wild-type seedlings in response to SNP (50 μM) treatment. (F) Activity of SOS4 and PDX3 in wild-type and nox1 mutant. In A–C, E, and F, 3-d-old well-germinated seedlings were transferred to the media supplemented with or without indicated concentrations of SNP for 15 d and collected for the HPLC (A–C) and enzyme activity (E and F) analyses. Data shown are means ± SD, n = 3.