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. 2014 Oct 27;111(45):16148–16153. doi: 10.1073/pnas.1409802111

Fig. 5.

Fig. 5.

Piezo1 directs neuronal–glial lineage choice in human neural stem cells. (A) SC23 hNSPCs display an increase in astrogenesis (GFAP+ cells) in the presence of 5 μM free GsMTx-4 (SI Methods). n = 3 independent experiments. (B) SC27 hNSPCs display a reduction in the percentage of Map2+ cells when differentiated in the presence of 5 μM free GsMTx-4. n = 4 biological repeats from three independent experiments. (C) SC27 hNSPCs transfected with 20 nM Piezo1 siRNA differentiate into astrocytes more efficiently than SC27 hNSPCs transfected with 20 nM control nontargeting (nonT) siRNA. n = 3 biological repeats from two independent transfection experiments. (D) SC27 hNSPCs transfected with 20 nM Piezo1 siRNA show reduced neurogenesis as compared with cells transfected with 20 nM control nontargeting siRNA. n = 3 biological repeats from two independent transfection experiments. (Scale bars: 20 μm.) ***P < 0.001 with two-sample t test. See also Fig. S7.