Fig. 4.

Homodimerization of A10TmCp-6H in the DPC micelle. (A) Plot of FS fluorescence quenching (F/F₀) as a function of the molar ratio of TMR-tri-NTA of FS-tri-NTA. F/F₀ values expected for a dimer (black dashed line), a trimer (green), and a tetramer (pink) are calculated according to (24, 27). (B) Plot of FRET efficiency (E) as a function of A10TmCp-6H concentration. A mixture of A10TmCp-6H/FS-tri-NTA/TMR-tri-NTA at a molar ratio of 2/1/1 was serially diluted and the FRET efficiency at each dilution measured. The plot was fitted to a linked equilibria of A10TmCp-6H dimerization and its binding to tri-NTA fluorophores (dashed line), with a dimerization constant of 7.2 ± 2.0 nM.