Fig. 6.

DiPal-A10Cp, but not A10Cp, forms an oligomer in DPC micelles and inhibits dimerization of A10TmCp-6H. (A) CD spectra of DiPal-A10Cp (blue) and A10Cp (brown) in the DPC micellar solution (10 mM DPC, 20 mM Tris·HCl, 100 mM NaCl, and 1 mM DTT, pH 8.0) at 20 °C. (B) Fluorescence emission spectra of 10 nM FS-tri-NTA (green), 100 nM TMR-tri-NTA (pink), and the mixture of both in complex with 200 nM DiPal-A10Cp-6H (blue) in the DPC micellar solution. (C) Plots of FS fluorescence quenching (F/F₀) vs. the molar ratio of added protein to A10TmCp-6H in DPC. Stock of DiPal-A10Cp (blue), A10Cp (brown) or lysozyme (black, as control) was added individually to the mixture of A10TmCp-6H with tri-NTA fluorophores, and the FS fluorescence intensities were measured as described in SI Materials and Methods.