Table 1.

Binding affinity of tri-NTA to A10Cp and A10TmCp in 10 mM DPC, 20 mM Tris·HCl, 100 mM NaCl, 1 mM DTT, and 1 µM NiSO₄, pH 8.0

Fluorophore	Protein	KNTA (nM)
FS-tri-NTA	A10Cp-6H	18 ± 2
TMR-tri-NTA	A10Cp-6H	16 ± 3
FS-tri-NTA	A10TmCp-6H	8 ± 2
TMR-tri-NTA	A10TmCp-6H	12 ± 4