Cladogram showing numbers of MAT-like loci. Species named in bold have been shown experimentally to switch mating types (3, 4, 12–18). Saccharomycotina, Pezizomycotina, and Taphrinomycotina are subphyla within phylum Ascomycota (19). Saccharomycetaceae, the CTG clade, and the methylotrophs are clades within the Saccharomycotina. The tree topology is based on refs. 20–26.