Table 1.
Fold change in expression of P. pastoris MAT loci determined by qRT-PCR
Gene | Ratio GS190(a)/GS115(α)* | Ratio GS115(α)/GS190(a)* | Ratio GS190(a)/SHY15(a/α)* | Ratio GS115(α)/SHY15(a/α)* |
a1 | 0.75 (0.57–0.97) | — | 3.36 (2.57–4.41) | 4.50 (3.86–5.24) |
a2 | 4.38 (3.41–5.62) | — | 2.83 (2.20–3.63) | 0.65 (0.47–0.89) |
α1 | — | 1.82 (1.19–2.77) | 0.66 (0.43–1.00) | 1.20 (1.03–1.39) |
α2 | — | 15.03 (13.18–17.15) | 0.11 (0.06–0.20) | 1.61 (1.41–1.84) |
n = 2-ΔΔCt.
Values in parentheses show ± SD; see SI Appendix, Table S1 for details of calculations.