Fig. 2.

Metagene analysis. (A) Global redistribution of ribosome density through the length of the genes in macrolide-treated cells. Highly expressed genes were length-normalized, split into an equal number of length segments, and ribosome density within each segment was computed as a fraction of the total density within the gene. Cumulative ribosome density for each segment across all of the ORFs was plotted against normalized gene length. (B) A limited number of genes show increased ribosome occupancy of the early codons in macrolide-treated cells. The combined ribosome density within codons 2–15 of common highly expressed genes in the ERY/control (n = 2,056) or TEL/control (n = 1,922) samples was computed, and the fraction of genes (%) showing a specific increase in the ratios between antibiotic-treated and drug-free samples was plotted.