Skip to main content
. 2014 Oct 28;111(45):E4822–E4831. doi: 10.1073/pnas.1415271111

Fig. 2.

Fig. 2.

TorA and LAP1 or LULL1 form a 1:1 stoichiometric complex. (A) Complex of MBP-tagged TorA Δ49EQ and LAP1LD or LULL1LD was analyzed by size exclusion chromatography. Elution positions of size markers are indicated by arrows. Elution fractions were subjected to SDS/PAGE, followed by colloidal blue staining. EQ, TorA E171Q Walker B mutant. (B) Size exclusion chromatography-purified complexes were incubated at room temperature for 10 min in the absence or presence of 0.1% (wt/vol) glutaraldehyde (GA) and resolved by gradient SDS/PAGE (6–9%), followed by silver staining.