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. 2014 Oct 28;111(45):E4822–E4831. doi: 10.1073/pnas.1415271111

Fig. 5.

Fig. 5.

Mutations of LAP1 and LULL1 Arg residues do not affect Torsin binding. (A) LAP1 and LULL1 Arg mutants interact with TorA in vivo. The 293T cells were transfected with TorA E171Q and the indicated LD constructs, lysed with Nonidet P-40 (Roche), and immunoprecipitated using an HA antibody. Input controls and immunoprecipitates were resolved by SDS/PAGE and blotted with the indicated antibodies. IB, immunoblot. (B) LDs of LAP1 or LULL1 were incubated at 30 °C in the absence or presence of equal molar TorA E171Q and 2 mM ATP. Protein complex formation was analyzed by size exclusion chromatography. Elution positions of size markers are indicated by arrows. Elution fractions were subjected to immunoblotting using the indicated antibodies. (C) Identical analysis for LAP1LD R563A or LULL1LD R449A. (D) Identical analysis for LAP1LD R563E or LULL1LD R449E.