Fig. 3.

Expression of PRV gD in porcine cells leads to CD112 degradation and reduced killing by porcine primary NK cells. (A) mRNA expression, assessed by RT-PCR, of CD112 and CD155 in porcine SK cells and of DNAM-1 in primary porcine NK cells. (B and C) Porcine SK cells (B) or primary porcine epithelial cells (C) were mock infected or infected for 12 h with WT or gDnull PRV and subsequently analyzed by Western blotting for expression of CD112, gB, gC, gD, and tubulin. (D) SK cells were mock infected or infected with WT or gDnull PRV and subsequently incubated with primary porcine NK cells at a target:effector ratio of 1:25 for 4 h. Viability was assessed by flow cytometry using propidium iodide. Data represent mean + SEM of three independent repeats (***P < 0.001).