Fig. 1.

A 70-kb transgene overexpresses Dlk1 in an authentic isoform-specific and spatiotemporal manner. (A) Real-time quantitative PCR (RT qPCR) of total Dlk1 transcripts in adult tissue. (B) Western blotting of an intracellular epitope of DLK1 in WT tissues (Left), ∼50 kDa both membrane-bound and cleaved ectodomain in pituitary (pit), whole e16 embryo (emb), and adrenal (adr); <20 kDa band is the membrane-bound remnant of cleavage. (Right) Relative protein expression between WT and WT/TG animals (70C line) in pit and adr samples (run on separate gels). (C) Serum levels of cleaved DLK1 ectodomain in adult mice from three transgenic lines and WT measured by ELISA (females at 6 mo of age, mean ± SEM). Numbers above the columns show concentrations relative to WT. WT, n = 10; 70A, n = 4; 70B, n = 3; 70C, n = 9.