Fig. 6.

Muscle fuel use and energy expenditure are altered in Dlk1-overexpressing mice. (A) RT qPCR of muscle genes in WT and WT/TG animals fed a HFD at 24 wk, measured by WT/TG (70C) male mice fasted for 16 h, n = 7 per genotype, on mixed soleus/gastrocnemius muscle. (B–D) Indirect calorimetry on mice fed a HFD, average respiratory exchange ratio (RER) (B), oxygen consumption (C), and CO₂ production (D) after a 16-h fast in WT and WT/TG mice, presented per gram body mass. n = 7 WT and 9 WT/TG. (E) Body temperature evaluation performed on mice in B–D. Values are mean ± SEM. All comparisons between WT and WT/TG (A–E) were performed by Mann-Whitney U test, *P < 0.05, **P < 0.01.