Fig. 3.

Mapping of the 3′ and 5′ extremities of pre-rRNA by circular RT-PCR with cDNA reverse transcribed with the 25c primer. (A and B) Increased accumulation of 32S rRNA in atprmt3-2 determined by PCR with r1/r2 (A) and r3/r2 primers (B). (C) Increased accumulation of 27SB intermediates in atprmt3-2 checked by PCR with r4/r2 primers. Negative images of ethidium bromide-stained 1.5% (wt/vol) agarose gels are shown. (A–C) Sizes of DNA markers (bp) are indicated on the left in A. (D) The diagram illustrates the amplified PCR fragments. For each fragment, the number of clones obtained from wild-type and atprmt3-2 samples is indicated on the right. The number of polyadenylated clones is marked in parentheses. –, no identification; Poly(A), template reverse transcribed from oligo-dT purified RNA; Total, template reverse transcribed from total RNA.