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. 2014 Oct 28;111(45):16190–16195. doi: 10.1073/pnas.1412697111

Fig. 4.

Fig. 4.

Mapping of 3′ and 5′ extremities of pre-18S rRNAs by circular RT-PCR with cDNA reverse transcribed with the 18c primer. (A) Striking decrease of P-A3 intermediates in atprmt3-2 checked by PCR with r5/r6 primers. (B and C) Significant increase of 18S-A3 intermediates in atprmt3-2 validated by PCR with r5/r7 (B) and r5/r8 (C). (D) The diagram illustrates the amplified PCR fragments. For each fragment, the number of clones obtained from wild-type and atprmt3-2 samples is indicated on the right. The number of polyadenylated clones is marked in parentheses. –, no identification; Poly(A), template reverse transcribed from oligo-dT purified RNA; Total, template reverse transcribed from total RNA.