Figure 3.

Relative enzymatic rates before and after partial ClpA autodegradation. Error bars are the standard error in the mean for three trials. A) ATPase rates, normalized to the rate of ClpA at t=0. White bars: ATPase rate of ClpA alone in the absence of ClpP at t=0. Dashed bars: ATPase rate of ClpA upon addition of ClpP. Gray bars: ATPase rate of ClpA after a five minute-incubation at 37°C with ClpP. Black bars: ATPase rate of ClpA after a five minute-incubation at 37°C in absence of ClpP. Note that the minimum relative ATPase rate on this plot is 0.4. B) Caseinolysis rates, normalized to the rate of wild-type ClpA prior to autodegradation. White bars: caseinolysis rate of ClpP immediately after addition of ClpA. Gray bars: caseinolysis rate of ClpP after five minutes incubation with ClpA at 37°C. C) Peptidolysis rates, normalized to the rate of ClpP in the absence of ClpA. White bars: peptidolysis rate of ClpP immediately after addition of ClpA. Gray bars: peptidolysis rate of ClpP after a five minute-incubation with ClpA at 37°C.