Table 1. Oligonucleotides used in this study.
| Primer | Sequence (5′-3′)a | Structureand/or positionb |
| HC2590-F | GTTAACACTGGAAAGAAACCAACCACAGTG | 2562 to 2591 |
| HC2590-R | TGTACACTATTTTGGGAGTCCGATCG | 3765 to 3740 |
| HC2776-F | CACTTAAGGTTACAGCTGCGGTGGCCGCCTCGATG | 2758 to 2792 |
| HC2776-R | CATCGAGGCGGCCACCGCAGCTGTAACCTTAAGTG | 2792 to 2758 |
| HC-F8 | TGGGATGGAGGTGAAGCAGA | 3486 to 3505 |
| HC-R3 | AAGGGCTGCCTCACAACTATGG | 3911 to 3892 |
| HC2590-ACG-F | GGTGGCCGCCACGATGCGGACTCGAAACCCTGGAGCAAAC | 2777 to 2816 |
| HC2590-ACG-R | CCGCATCGTGGCGGCCACCGGAGCTGTAACCTTAAGTGTTG | 2795 to 2755 |
| HC2590-ACA-F | GGTGGCCGCCACAATGCGGACTCGAAACCCTGGAGCAAAC | 2777 to 2816 |
| HC2590-ACA-R | CCGCATTGTGGCGGCCACCGGAGCTGTAACCTTAAGTGTTG | 2795 to 2755 |
| HC-F12 | taatacgactcactataGGGAAAATTGCTTTATCATAACC | T7, 2438 to 2460 |
| HC-R12 M | GCTCCAGGGTTTCGAGTCCGCATTGTGGTTGGTTACTTTCCA | 2814 to2791, 2589 to 2570 |
| HC-F16 | ATGCGGACTCGAAACCCTGGAGC | 2791 to 2814 |
| HC-F5 | ggatccATGCTCTCACATGCTTTCTC | BamHI, 41 to 60 |
| HC-R5 | ctgcagTCACGGGCGAGTACCCCTGA | PstI, 670 to 650 |
a
Upper case, upper case with underlined and lower case refer to HCRSV cDNA nucleotides, mutated HCRSV cDNA nucleotides and added sequences (T7 promoter or restriction enzyme sites), respectively.
b
Numbers correspond to HCRSV nucleotide positions.