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. 2014 Nov 17;9(11):e113250. doi: 10.1371/journal.pone.0113250

Figure 6. Long Pepper Extact (PLX) Activates the Extrinsic & Intrinsic Pathways of Apoptosis.

Figure 6

Following treatment with 0.10 mg/ml PLX, at indicated time points, BxPc-3 cells were collected, washed and incubated with lysis buffer to obtain cell lysate. The cell lysate was incubated with caspase substrates, specific to each caspase (3, 8 and 9) and incubated for an hour. Fluorescence readings were obtained using a spectrofluorometer. An average of 6 readings per well and a minimum of three wells were run per experiment. The results here are reported as activity per µg of protein (in fold) and the average of three independent experiments is shown. (B) The reduction in viability was caspase independent, as a pan-caspase inhibitor, Z-VAD-fmk could not prevent the loss of viability induced by PLX treatment in colon and pancreatic cancer cells. Absorbance was read at 450 nm and expressed as a percent of the control. Values are expressed as mean ± SD from quadruplicates of 3 independent experiments. **P<0.0001.