Table 1. Analysis of five well-known piperamides and crude long pepper extract at a flow rate of 1.0 mL/min with a mobile phase constituted of H2O and methanol.
Time (mins) | H20 (%) | MeOH (%) |
0.0 | 37.5 | 62.5 |
15.0 | 35.0 | 65.0 |
35.0 | 0.0 | 100.0 |
45.0 | 0.0 | 100.0 |
46.0 | 37.5 | 62.5 |