Fig. 1.

Epitope Map of P99βL. The total number of predicted epitopes (y-axis) that start at a given amino acid is mapped onto the primary sequence of P99βL (x-axis). Using a Propred threshold of 5 %, epitopes were predicted for MHC II alleles DRB1*0101, 0301, 0401, 0701, 0801, 1101, 1301, and 1501 (i.e., max score for any position is 8). Sites of IP² mutations are indicated with arrows and residue numbers. Inset: Predicted epitopes mapped onto the P99βL peptide backbone (PDB 1XX2 chain A). The density of predicted epitopes is indicated by size and color gradients. Thick tubes indicate a high density of overlapping epitopes, while thin white tubes indicate no epitopes in that protein segment. Residues targeted with mutations are rendered as pink ball and sticks. Rendered with PyMOL [43]