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. Author manuscript; available in PMC: 2014 Nov 17.
Published in final edited form as: Curr Biol. 2010 Feb 11;20(4):300–309. doi: 10.1016/j.cub.2009.12.055

Figure 6.

Figure 6

Circadian clocks in Gr5a neurons regulate feeding, food storage and activity. (a, b) Relative food intake was measured after 24 h of feeding by quantifying the amount of blue food dye ingested (a) or by measuring the food consumed using the CAFE assay (b) (see Experimental Procedures). Flies carrying both the Gr5a-Gal4 and UAS-cycDN transgenes show significantly (p < 0.02) increased feeding compared to control flies containing either the UAS-cycDN or the Gr5a-Gal4 transgene. (c) The body weight of flies carrying both the Gr5a-Gal4 and UAS-cycDN transgenes was not different (p > 0.50) than control flies bearing the UAS-cycDN or Gr5a-Gal4 transgenes. (d) Overall activity was measured as the number of times flies crossed an infrared light beam during a 24 h period. Flies carrying both the Gr5a-Gal4 and UAS-cycDN transgenes show significantly (p < 0.001) increased activity compared to control flies containing either the UAS-cycDN or the Gr5a-Gal4 transgene. (e) Glycogen levels are significantly (p < 0.04) higher in flies carrying both the Gr5a-Gal4 and UAS-cycDN transgenes than control flies bearing the UAS-cycDN or Gr5a-Gal4 transgenes. (f) Triglyceride levels are significantly (p < 0.001) higher in flies carrying both the Gr5a-Gal4 and UAS-cycDN transgenes than control flies bearing the UAS-cycDN or Gr5a-Gal4 transgenes. Error bars represent +/− S.E.M. Increased feeding and activity in flies lacking clocks in Gr5a neurons is not uniform over the circadian cycle (Table S1).