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. 2014 Sep 10;16(8):644–655. doi: 10.1016/j.neo.2014.07.007

Figure 2.

Figure 2

KAT6A knockdown reduces cell growth in 8p11-p12 amplicon bearing cell lines. A. RT-PCR results showing knockdown of KAT6A at the mRNA level in SUM-52 and SUM-225 cells transduced with lentivirus using shRNA1 and shRNA3. Below is a corresponding western blot showing knockdown of KAT6A at the protein level. B. RT-PCR data showing knockdown of KAT6A at the mRNA level in MCF10A and SUM-149 cells using lentiviral vectors shRNA1 and shRNA3. Below is a corresponding western blot showing knockdown of KAT6A protein. C - F. SUM-52, SUM-225, MCF10A, and SUM-149 control and KAT6A knockdown cells, respectively, were plated in triplicate and assayed for growth. Cell counts were taken on days 1, 4 and 7.