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. 2014 Sep 20;16(9):679–693. doi: 10.1016/j.neo.2014.08.001

Figure 2.

Figure 2

Mitochondrial membrane potential is lost in response to transient expression of HGV-Apoptin. A) Mitochondrial staining of HCT116 cells transfected with GFP-HGV-APT or the corresponding pEGFPC1 control plasmid. Cells were stained with TMRM dye for 15 min, then fixed and counterstained with DAPI. B) Monitoring of mitochondrial membrane potential (Ψm) in HCT116, either transfected with the HGV-Apoptin tagged to GFP: GFP-HGV-APT or the control plasmid: pEGFPC1, for the indicated time periods. Ψm was determined using TMRM dye, which shows a decrease in the red fluorescence upon loss of the mitochondrial potential. C) Monitoring of the mitochondrial potential in Jurkat WT and Jurkat-FADD-DN from 12 to 96 h post-transfection with GFP-HGV-APT and pEGFPC1.