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. 2014 Sep 20;16(9):679–693. doi: 10.1016/j.neo.2014.08.001

Figure 3.

Figure 3

HGV-Apoptin triggers the release of pro-apoptotic mitochondrial proteins. A) HGV-Apoptin induces cyt c release from transformed cells. MCF7-cyt c-GFP cell line was transiently transfected with FLAG-HGV-APT and the corresponding FLAG control vector, at 48 h post-transfection cells were fixed, stained with a primary rabbit anti-FLAG and secondary Red-X anti-rabbit antibody and counterstained with DAPI. B) The cellular localization of AIF in HCT116 cells as determined by confocal microscopy. HCT116 cells were transiently transfected with GFP-HGV-APT and the corresponding pEGFPC1 control plasmid, after 48 h cells were fixed, stained with a primary rabbit anti-AIF and secondary Red-X anti-rabbit antibody and counterstained with DAPI for nuclear visualization (the non-transfected cells along with p-EGFPC1 transfected cells were used as negative controls).