Skip to main content
. 2014 Sep 20;16(9):679–693. doi: 10.1016/j.neo.2014.08.001

Figure 5.

Figure 5

HGV-Apoptin mediated cell death is caspase-dependent. A) Quantification of cell survival as percentage of PO-PRO/7AAD double negative cells in the GFP-positive population from 24 to 120 h post-transfection. Jurkat cells were transfected either with GFP-HGV-APT or with pEGFPC1 control plasmid and then either left untreated or incubated with 25 μM of QVD-oph and then stained with PO-PRO/7AAD. B) Quantification of cell survival as percentage of PO-PRO/7AAD double negative cells in the GFP-positive population from 24 to 96 h post-transfection. Wild-type caspase-3 deficient MCF7 cells and MCF7 cells expressing caspase-3 (MCF7/C3) were transiently transfected either with GFP-HGV-APT or with pEGFPC1 control plasmid and then either left untreated or incubated with 25 μM of QVD-oph. Cells were then stained with PO-PRO/7AAD and analyzed by flow cytometry.