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. 2014 Sep 20;16(9):679–693. doi: 10.1016/j.neo.2014.08.001

Figure 6.

Figure 6

HGV-Apoptin induced apoptosis is regulated by the anti- and pro-apoptotic BCL-2 family members. A) Quantification of cell survival as percentage of PO-PRO/7AAD double negative cells in the GFP-positive population from 24 to 96 h post-transfection. MCF7 cells and MCF7-BCL-XL cells were transfected with GFP-HGV-APT or the corresponding control plasmid pEFPC1 and stained with PO-PRO/7AAD. B) Monitoring of the mitochondrial membrane potential in MCF7 WT and MCF7 BCL-XL cells after transfection with either GFP-HGV-APT or the control plasmid using the TMRM dye. C) Quantification of cell survival in Jurkat wild type and Jurkat-BCL-XL as percentage of PO-PRO/7AAD double negative cells in the GFP-positive population from 24 to 96 h post-transfection. Cells were transfected by electroporation with GFP-HGV-APT or the corresponding control plasmid. D) Monitoring of the mitochondrial membrane potential in Jurkat WT and Jurkat BCL-XL after transfection with either GFP-HGV-APT or the control plasmid using the TMRM dye. E) Quantification of apoptosis in the wild-type murine embryonic fibroblasts (MEF-WT) and MEF BAX and BAK deficient cells (MEF-BAX-BAK-KO). Cells were transfected with GFP-HGV-Apoptin or the corresponding control plasmid pEFPC1. Cell survival was quantified by flow cytometry after PO-PRO/7AAD staining as percentage of PO-PRO/7AAD double negative cells in the GFP-positive population of cells. F) Monitoring of the mitochondrial membrane potential in MEF-WT and MEF-BAX-BAK-KO using the TMRM dye staining. Due to space constrain “BCL-XL” was labeled in some figures as “BCLxl”.