Figure 7.

HGV-Apoptin induced apoptosis is independent from p53 signal. A) p53 deficient HCT116 (HCT116-p53^−/−) and the wild type (HCT116-WT) colon carcinoma cells were transfected with pEGFPC1 or GFP-HGV-APT. The cells were fixed and stained with DAPI 48 h post-transfection for the detection of the nuclear morphology; arrows indicate apoptotic cells expressing GFP-HGV-APT. B) Quantification of apoptosis in HCT116-WT and HCT116-p53^−/− cells using laser-scanning cytometry. Cells were fixed and stained with DAPI then analysed by laser scanning cytometry, cells with a high blue max-pixel values (condensed chromatin) are considered apoptotic. C) Western blot data confirming that HCT116-p53^−/− lack p53.