Table 1.

Specific primers designed in the present study to detect the three microsporidians found in G. pulex

Primer	Sequence (5′ - 3′)	Annealing Temperature
Mspec1 F	CAT CAA CTA ACT TTG GGA AAC TAA G	57°C
Mspec1 R	TGG CCT CCC ACA CAT TCC GAG TG
Mspec2 F	GGC GAT CTA ACC TCG GCA TCG GAT AAC C	64°C
Mspec2 R	TGG CTT CCC ACC CAT TCC GAG C
Mspec 3 F	CAG TAA TGT TGC GAT GAT TTG GTC	55°C
Mspec3 R	CAG TAA ATA CTC CAC AGT ATC TTA C