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. Author manuscript; available in PMC: 2015 Apr 23.
Published in final edited form as: Nature. 2014 Oct 23;514(7523):498–502. doi: 10.1038/nature13814

Figure 4. CLEC-2+ dendritic cells are required for LN swelling during adaptive immune responses.

Figure 4

a) Mass of skin draining LNs from CD11cΔCLEC-2 mice and Creneg littermate controls, >24 weeks old. Data are normalised to average LN mass of Creneg control. Each data point represents 1 LN. Mann Whitney U test (****, p< 0.00001). b) LN mass (mg) of draining and non-draining LNs from 8-12 week old CD11cΔCLEC-2 mice and Creneg littermates (control) immunised with OVA/CFA (day 7). Each point represents a LN. Data from 3 experiments. 2-way ANOVA, Tukey’s multiple comparisons (**, p< 0.001). c) Gap analysis of draining LNs from immunised control (top) and CD11cΔCLEC-2 mice (bottom), quantified on the right. p = 0.0001759 (radii> 8 μm), Fisher’s exact test. d) LN deformation following compression of whole LNs with 1.4N force. Each point represents one LN. p<0.05, 1-way ANOVA. e) LN mass of CD11cΔCLEC-2 mice and Creneg littermates (control) 7 days following CFA/OVA immunisation and treatment with 10 μg CLEC-2-Fc or PBS (days 1 and 3). Each point represents one LN, data from 3 experiments. 2-way ANOVA, Tukey’s multiple comparisons test (**, p< 0.001; ***, p< 0.0001; n.s., non-significant).