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. 2014 Sep 20;16(9):694–709. doi: 10.1016/j.neo.2014.07.011

Figure 2.

Figure 2

Knockdown of the MCMBP transcript has a direct effect on sister chromatid cohesion in different breast cancer cell lines. (A) Examples of metaphases wherein the sister chromatids are separated when the MCMBP transcript is deregulated, either transiently (MCF10A cell line) or in a stable manner (MDA-MB-231 cell line). Insets show higher magnification images of single sister chromatid pairs. For the MDA-MB-231 cells, control refers to the empty vector used for the lentiviral transfection, while for MCF10A cells, control refers to a pool of siRNAs designed not to target any gene in the human genome. (B) Quantification of disrupted metaphases for the analyzed cell lines. Disrupted metaphases refer to metaphases in which at least 70% of the chromosomes have totally separated sister chromatids. (C) qPCR analysis of the cultures showing depletion of the MCMBP transcript in the breast cancer cell lines.