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. 2014 Sep 20;16(9):694–709. doi: 10.1016/j.neo.2014.07.011

Figure 3.

Figure 3

Cellular analysis of MCF7 cells in which MCMBP is knocked down or overexpressed. (A) Flow cytometry profiles of cells with MCMBP knockdown in comparison with controls. Upon knockdown, a subpopulation of cells with DNA content higher than 4C appeared in the culture. (B) A correlation exists between multinucleation and reduction or absence of MCMBP protein expression in those types of cells, both in control and knocked down cultures. (C) Quantification of the multinucleated cells present in the different cultures. (D) Up-regulation of specific cell cycle markers on MCMBP knockdown. (E) On MCMBP overexpression, multinucleated cells and micronuclei (arrows) can be observed. (F) Quantification of the multinucleated cells present in the different cultures. (G) Up-regulation of specific cell cycle markers on MCMBP overexpression.