Figure 4.

mRNA expression of PLAC1 is elevated in NCOA3 overexpressing and ERα-positive human breast cancer samples. (A)NCOA3 and PLAC1 mRNA expression was examined by quantitative real-time RT-PCR in 48 human breast cancer samples. Relative expression of NCOA3 below 100 000 were considered to be ‘NCOA3 low’ (N = 25), whereas relative expression of NCOA3 over 100 000 were considered to be ‘NCOA3 high’ (N = 23). (B) Evaluation of PLAC1 expression in ERα-positive breast cancer samples with ‘NCOA3 low’ (N = 20) or ‘NCOA3 high’ (N = 13) status. (C) Evaluation of PLAC1 expression in ERα-negative breast cancer samples with ‘NCOA3 low’ (N = 5) or ‘NCOA3 high’ (N = 10) status. Statistical analysis was performed using two-tailed Student’s t-test. P ≤ 0.05 (*) was considered statistically significant.