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. 2014 Sep 26;3(5):735–742. doi: 10.1016/j.stemcr.2014.08.013

Figure 1.

Figure 1

Endogenous SPOT14 Protein Is Expressed in the Adult Hippocampus

(A) Endogenous SPOT14 protein revealed by antibody staining is confined to the SGZ and colocalizes with the NSPC markers SOX2 (red) and NESTIN (blue). Shown are representative images of a wt mouse at 2 m (left panel) and a magnification of the boxed area (right panel). Arrows point to SPOT14/SOX2/NESTIN-positive radial NSPCs, and arrowheads point to SPOT14/SOX2-positive nonradial NSPCs.

(B) SPOT14-GFP-expressing cells colabel with the immunohistochemical signal from endogenous SPOT14 protein. Shown are representative images of a SPOT14 reporter mouse at 2 m (left panel) and a magnification of the boxed area (right panel). Arrows point to SPOT14-GFP/SPOT14-antibody positive radial NSPCs, and arrowheads point to SPOT14-GFP/SPOT14 antibody-positive nonradial NSPCs.

Scale bars represent 50 μm (A and B, left) and 20 μm (A and B, right). GCL, granular cell layer.