Figure 7.

Generation of Oligodendrocytes Is Facilitated by Induction of let-7

(A) At 6 weeks of differentiation toward the oligodendrocyte lineage, OPC markers were identified by immunostaining and quantified in (B).

(B) Quantification of results presented in (A).

(C) Following at least 6 weeks of differentiation in either base or glial induction medium, mature oligodendrocytes were identified by immunostaining and quantified in (D). The bottom panels followed a glial induction protocol (Wang et al., 2013) and produced oligodendrocytes after just 6.5 weeks, again, only with let-7 induction at the NPC stage prior to directed differentiation. At 7.5 weeks, the number of oligodendrocytes produced was considerably larger. Scale bar, 100 mm.

(D) A quantification of oligodendrocytes in each condition at two time points in each well. Cells were judged as bona fide oligodendrocytes if positive for both O4 and MBP.