Figure 5.

IK6 Sensitizes Human GM Progenitors to GM-CSF and IL-3

(A) Effect of different human growth factors on 6-week cell outputs from IK6- and control-transduced human cells (mean ± SEM; four replicates each from two experiments).

(B and C) IL-3 and GM-CSF dose-response relationships for GM colony formation from IK6- and control-transduced human CD34⁺ cells (mean ± SEM; two replicates each from two experiments).

(D) Differences in levels of intracellular phosphoproteins in IK6- versus control-derived CD34⁻11b⁺ GM cells stimulated (or not) with GM-CSF or IL-3 (mean ± SEM of MFI ratios; six to eight experiments).

(E) Basal levels of intracellular phosphoproteins in IK6- versus control-derived CD34⁺ cells (mean ± SEM of MFI ratios from six to eight experiments).

(F) Representative flow cytometric profiles of phospho-CREB levels (solid lines) in IL-3-stimulated CD34⁺ cells derived from IK6- and control-transduced cells. Dotted lines show staining in the absence of the phospho-CREB antibody.

(G) Phospho-CREB response of CD34⁺ cells derived from IK6- and control-transduced cells to increasing concentrations of GM-CSF (mean ± SEM for normalized changes in phospho-CREB MFI values, four experiments).

MFI, median fluorescence intensity; FL, FLT3-ligand. ^∗p < 0.05; ^∗∗p < 0.01; ^∗∗∗p < 0.001.