Figure 6.

IK6 Activates CREB and Its Targets FOS and Cyclin B1 in CD34⁺ Human CB Cells

(A) IK6-stimulated gene expression in subsets of CD34⁺ cells from 3- to 4-week LTCs (mean ± SEM ratio of normalized transcript levels; seven experiments).

(B) Representative flow cytometric profile of intracellular FOS protein levels (solid lines) in CD34⁺ cells derived from IK6- and control-transduced cells in 3-week LTCs. Dotted lines indicate staining in the absence of anti-FOS antibody.

(C) Percentage differences in phosphoprotein levels (MFIs) in different subsets of CD34⁺ cells generated from IK6- and control-transduced cells cotransplanted into NSG mice (mean ± SEM, four mice). Phospho-ERK was not detected.

(D) FOS protein levels in progeny of IK6- and control-transduced cells cotransplanted into NSG mice 10 weeks previously (mean ± SEM, four mice).

(E) Representative flow cytometric plots of lymphoid progenitor (CD34⁺CD38⁺CD10⁺) cells from NSG mice cotransplanted 10 weeks previously with IK6- and control-transduced cells and then stained for intracellular Cyclin B1 protein.

(F) Percent Cyclin B1⁺ human cells in NSG mice cotransplanted with IK6- and control-transduced cells 10 weeks previously (mean ± SEM; four mice).

MFI, median fluorescent intensity; ^∗p < 0.05.