Figure 7.

IK6 Activates a B-Lineage Transcriptional Program Prematurely in Human CD34⁺CD38⁻ Cells

(A) Normalized transcript levels in subsets of human cells generated from IK6- versus control-transduced cells in four cotransplanted NSG mice (mean ± SEM).

(B) Differential enrichment of genes bearing specific transcription factor binding sites in a comparison of gene expression data from the CD34⁺CD38⁻ progeny of IK6- and control-transduced cells sorted from transplanted mice. “Up” indicates a positive enrichment. “Down” indicates a negative enrichment. “Mixed” indicates enrichment including both up- and downregulated genes.

(C) Unsupervised clustering and heatmap of human CREB target genes (500 genes showing strongest CREB binding in a chromatin immunoprecipitation sequencing study of human cells; Zhang et al., 2005) showing relative expression (Z score) in CD34⁺CD38⁻ cells.

(D) Unsupervised clustering and heatmap of 127 genes whose expression is ≥2-fold higher in normal human CD34⁺CD38⁺CD10⁺ B cell progenitors as compared to normal human CD34⁺CD38⁻ CB cells (Hystad et al., 2007) showing their relative expression (Z score) in CD34⁺CD38⁻ cells.

^∗p < 0.05.