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. Author manuscript; available in PMC: 2014 Nov 18.
Published in final edited form as: J Dairy Sci. 2012 Jul;95(7):3852–3864. doi: 10.3168/jds.2011-5251

Table 1.

Details of primer sequences used for QPCR experiments.

Gene symbol Forward and reverse primer sequences (5′→3′) Amplicon
length (bp)
Accession
number
Reference
Primers used for QPCR experiment
ß-actin 5′- GCAAATGCTTCTAGGCGGACT-3′
5′- CAATCTCATCTCGTTTTCTGC G-3′
85 BT030480 Pareek et al. 2005
IL-8 5′- GCTGGCTGTTGCTCTCTTG-3′
5′- AGGTGTGGAATGTGTTTTTATGC-3′
118 EU276073 Pareek et al. 2005
IL-6 5′-TGAGGGAAATCAGGAAAATGT -3′
5′-CAGTGTTTGTGGCTGGAGTG -3′
110 EU276071 Pareek et al. 2005
TLR4 5′- ACTGCAGCTTCAACCGTATC-3′
5′- TAAAGGCTCTGCACACATCA -3′
190 AB056444 Ibeagha-Awemu et
al. 2007
CXCL12 5′- ATTTAGGCCATGGAGGCTCT -3′
5′- GAAACTGTGCTGTGGCTTCA -3′
117 NM_001113174 -
SST 5′- TGGAGCCTGAAGATTTGTCC -3′
5′- GAAATTCTTGCAGCCAGCTT -3′
122 NM_173960 -
MX1 5′- ACAGATGCGTCAGTGGTCAG -3′
5′- TGGAGAGAGTTCAGGGATGG -3′
133 NM_173940 -
IL1β 5′- CAAGGAGAGGAAAGAGACA -3′
5′- TGAGAAGTGCTGATGTACCA G-3′
209 M37211 Konnai et al. 2003
Keratin 5 5′- CAAGGTCCTGGACACCAAGT-3′
5′- TCCAGCTGTCTCCTGAGGTT-3′
114 NM_001008663
TLR4 amplification and sequencing primers
TLR4
promoter
(Amplification)
5′- CAAGATTTCACAAAATTGGACAGTGCC -3′
5′- TACCTGTACGCAAGGGTCCCAA -3′
1870 NC_000070 -
TLR4
promoter
(Sequencing)
5′- TCCCTTGGACTGCAAGGAGATCCA -3′
5′- AGGCACAGGGAGAAGACAGCCA -3′
- NC_000070 -
TLR4
exon 1
5′- CCTCTCACTTCCTCTCCTCCCGTAAC -3′
5′- CCTCGGGCTGCCTGTTAATGC -3′
434 NM_174198 -
TLR4
exon 2
5′- GTGACTTATGATTGGATGGA -3′
5′- GTAAGATGTCTGTTGAGTGA -3′
458 NM_174198 -
TLR4
exon 3
5′- ATATCTGTGTGGAGACCTAGATGACTGGG -3′
5′- GAACACGCCCTGCATCCATCTG -3′
2414 NM_174198 -