Table 1.
Gene symbol | Forward and reverse primer sequences (5′→3′) | Amplicon length (bp) |
Accession number |
Reference |
---|---|---|---|---|
Primers used for QPCR experiment | ||||
ß-actin | 5′- GCAAATGCTTCTAGGCGGACT-3′ 5′- CAATCTCATCTCGTTTTCTGC G-3′ |
85 | BT030480 | Pareek et al. 2005 |
IL-8 | 5′- GCTGGCTGTTGCTCTCTTG-3′ 5′- AGGTGTGGAATGTGTTTTTATGC-3′ |
118 | EU276073 | Pareek et al. 2005 |
IL-6 | 5′-TGAGGGAAATCAGGAAAATGT -3′ 5′-CAGTGTTTGTGGCTGGAGTG -3′ |
110 | EU276071 | Pareek et al. 2005 |
TLR4 | 5′- ACTGCAGCTTCAACCGTATC-3′ 5′- TAAAGGCTCTGCACACATCA -3′ |
190 | AB056444 | Ibeagha-Awemu et al. 2007 |
CXCL12 | 5′- ATTTAGGCCATGGAGGCTCT -3′ 5′- GAAACTGTGCTGTGGCTTCA -3′ |
117 | NM_001113174 | - |
SST | 5′- TGGAGCCTGAAGATTTGTCC -3′ 5′- GAAATTCTTGCAGCCAGCTT -3′ |
122 | NM_173960 | - |
MX1 | 5′- ACAGATGCGTCAGTGGTCAG -3′ 5′- TGGAGAGAGTTCAGGGATGG -3′ |
133 | NM_173940 | - |
IL1β | 5′- CAAGGAGAGGAAAGAGACA -3′ 5′- TGAGAAGTGCTGATGTACCA G-3′ |
209 | M37211 | Konnai et al. 2003 |
Keratin 5 | 5′- CAAGGTCCTGGACACCAAGT-3′ 5′- TCCAGCTGTCTCCTGAGGTT-3′ |
114 | NM_001008663 | |
TLR4 amplification and sequencing primers | ||||
TLR4 promoter (Amplification) |
5′- CAAGATTTCACAAAATTGGACAGTGCC -3′ 5′- TACCTGTACGCAAGGGTCCCAA -3′ |
1870 | NC_000070 | - |
TLR4 promoter (Sequencing) |
5′- TCCCTTGGACTGCAAGGAGATCCA -3′ 5′- AGGCACAGGGAGAAGACAGCCA -3′ |
- | NC_000070 | - |
TLR4 exon 1 |
5′- CCTCTCACTTCCTCTCCTCCCGTAAC -3′ 5′- CCTCGGGCTGCCTGTTAATGC -3′ |
434 | NM_174198 | - |
TLR4 exon 2 |
5′- GTGACTTATGATTGGATGGA -3′ 5′- GTAAGATGTCTGTTGAGTGA -3′ |
458 | NM_174198 | - |
TLR4 exon 3 |
5′- ATATCTGTGTGGAGACCTAGATGACTGGG -3′ 5′- GAACACGCCCTGCATCCATCTG -3′ |
2414 | NM_174198 | - |