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The rationale of BioVector. The LR reaction among GEC, PEC and BDV shows the basic principle of BioVector, in which a gene and a promoter in individual entry clones are positioningly and directionally joined together in a destination vector. attL1/2/3/4, attR1/2/3/4, and attP1/2/3/4, recombination sites; ccdB, a negative selection marker for DH5α; LR Clonase II®, recombination enzyme from Invitrogen.
