Table 1.
Vector name | Tag and its position | Restriction enzyme sites at both ends of tags | ABRC stock number✶ |
---|---|---|---|
Fu28 |
C-GFP |
Sac I |
CD3-1822 |
Fu30 |
N-GFP |
Bgl II |
CD3-1823 |
Fu41 |
N-YFP |
Bgl II, Xba I |
CD3-1824 |
Fu42 |
C-YFP |
EcoR I, Sac I |
CD3-1825 |
Fu43 |
N-CFP |
Bgl II, Xba I |
CD3-1826 |
Fu44 |
C-CFP |
EcoR I, Sac I |
CD3-1827 |
Fu45 |
N-mRFP |
Bgl II, Xba I |
CD3-1828 |
Fu46 |
C-mRFP |
EcoR I, Sac I |
CD3-1829 |
Fu47 |
N-3MYC |
Bgl II |
CD3-1830 |
Fu48 |
C-3MYC |
Sac I |
CD3-1831 |
Fu49 |
N-3FLAG |
Bgl II |
CD3-1832 |
Fu50 |
C-3FLAG |
Sac I |
CD3-1833 |
Fu55 |
N-3HA |
Bgl II |
CD3-1834 |
Fu56 |
C-3HA |
EcoR I |
CD3-1835 |
Fu58 |
N-GST |
Bgl II, Xba I |
CD3-1836 |
Fu59 |
N-StrepII |
Bgl II |
CD3-1837 |
Fu60 |
N-NLS |
Bgl II |
CD3-1838 |
Fu61 |
C-NES |
Sac I |
CD3-1839 |
Fu62 |
C-GUS |
EcoR I, Sac I |
CD3-1840 |
Fu63 |
C-LUC |
EcoR I, Sac I |
CD3-1841 |
Fu64 |
N-FLAG; C-YFPc |
Bgl II for FLAG; |
CD3-1842 |
EcoR I, Sac I for YFPc | |||
Fu65 |
N-MYC; C-YFPn |
Bgl II for MYC; |
CD3-1843 |
EcoR I, Sac I for YFPn | |||
Fu66 |
N-YFPc; C-HA |
Bgl II, Xba I for YFPc; |
CD3-1844 |
Sac I for HA | |||
Fu67 |
N-YFPn; C-MYC |
Bgl II, Xba I for YFPn; Sac I for MYC |
CD3-1845 |
Fu79 | Tag free | CD3-1846 |
✶All vectors were delivered to ABRC (http://www.arabidopsis.org/). “N” or “C” indicates that the position of a tag is at the “N” or “C” terminus of a target gene, “n” or “c” for the “n” or “c” half part of YFP fluorescence protein. All vectors contain recombination sites of attL1 and attL2 and have the chloramphenicol selection marker in E. coli. All the sequences are showed in Additional file 1.