FIG 2 .

Single-molecule imaging of HaloTag-labeled SusG in glucose-grown B. thetaiotaomicron. Diffraction-limited images (A and C) (white) and reconstructed superresolution localization images (B and D) (red) of TMR-HaloTag-labeled SusG in fixed and live cells, respectively. The superresolution images in panels B and D are merged with phase-contrast cell images (black). All images were constructed by stacking imaging frames obtained over time at 10 frames per s. The fixed-cell images show a few discrete spots representing stationary SusG molecules, while the live-cell images include many spots that correspond to a few SusG molecules that were moving on the membrane over time.